FIG. 2.

Combination treatment with a nucleoside inhibitor reduces the emergence of resistant colonies. Huh7 cells that stably maintain a GT 1b subgenomic replicon carrying the neoR gene were incubated with one or two specific HCV inhibitors at 1, 10, and 15 times the EC₅₀ in the presence of G418. After selection for 3 weeks, the remaining cells were fixed and stained with crystal violet. The replicon cells were treated with R1479 and HCV-796 (A), R1479 and VX-950 (B), PSI-6130 and HCV-796 (C), or PSI-6130 and VX-950 (D), and a representative example of at least four independent experiments is shown.