TABLE 3.

Genotypic characterization of NS5B or NS3 from replicons after single or combination selection

Selectiona	Mutation(s)b
	NS5B	NS3
Untreated	None	None
1× R1479	None	None
1× PSI-6130	None	NDg
1× NM107	None	ND
1× HCV-796	None	ND
1× HCV-796/1× R1479	None	ND
10× HCV-796	C316C/Yc, S365S/Ad	ND
10× HCV-796/1× R1479	C316Y	ND
10× HCV-796/1× PSI-6130	ND	ND
15× HCV-796	C316Y	ND
15× HCV-796/1× R1479	C316Y	ND
15× HCV-796/1× PSI-6130	C316Y	ND
1× VX-950	None	None
1× VX-950/1× R1479	None	None
1× VX-950/1× PSI-6130	None	None
10× VX-950	None	A156T/Se
10× VX-950/1× R1479	None	A156T/S
10 × VX-950/1× PSI-6130	None	T54 T/Af, A156A/S
15× VX-950	None	A156T/S
15× VX-950/1× R1479	None	A156T/S
15× VX-950/1× PSI-6130	None	A156S

^aReplicon cells were either untreated or selected with 1, 10, or 15 times the EC₅₀ of the indicated compounds.

^bDirect sequencing from PCR products, representing the major population (in one-letter amino acid code). Only amino acid substitutions known to confer resistance are reported.

^cThe mutation C316Y is a known resistance mutation for HCV-796.

^dThe mutation S365A is a known resistance mutation for HCV-796.

^eThe mutations A156T and A156S are known resistance mutations for VX-950.

^fThe mutation T54A is a known resistance mutation for VX-950.

^gND, not determined.