TABLE 1.
pncA gene sequences and PZA drug susceptibilities for 71 clinical isolates belonging to the M. tuberculosis complex
| No. of isolates | Susceptibility to PZAa | pncA gene description or nucleotide change(s)b | PZase description or amino acid change(s) |
|---|---|---|---|
| 34 | S | wt | wt |
| 1 | S | C insertion between nt −3 and −2 | wt |
| 1 | S | G insertion between nt −33 and −32 | wt |
| 1 | S | C(195)→T | Ser(65)→Ser |
| 1 | R | wt | wt |
| 1 | R | A(−11) →G | wt |
| 1 | R | T(17)→C | Ile(6)→Thr |
| 1 | R | C deletion at nt 99 | Frameshift |
| 1b | R | G(117)→A, C(169)→G | Ala(39)→Ala, His(57)→Asp |
| 3 | R | A(139)→G | Thr(47)→Ala |
| 2 | R | C(153)→A | His(51)→Gln |
| 1 | R | C(160)→T | Pro(54)→Ser |
| 1 | R | C(161)→T | Pro(54)→Leu |
| 1c | R | C(169)→G | His(57)→Asp |
| 1 | R | T(172)→C | Phe(58)→Leu |
| 1 | R | C(174)→G | Phe(58)→Leu |
| 1 | R | C(195)→T, GC insertion between nt 528 and 529 | Ser(65)→Ser, frameshift |
| 1 | R | T(199)→C | Ser(67)→Pro |
| 1 | R | T(202)→C | Trp(68)→Arg |
| 1 | R | T(269)→G | Ile(90)→Ser |
| 1 | R | T(307)→C | Tyr(103)→His |
| 1 | R | G(368)→C | Arg(123)→Pro |
| 1 | R | G(373)→T | Val(125)→Phe |
| 2 | R | T(374)→A | Val(125)→Asp |
| 1 | R | GG insertion between nt 391 and 392 | Frameshift |
| 1 | R | G(394)→A | Gly(132)→Ser |
| 1 | R | G(395)→C | Gly(132)→Ala |
| 1 | R | G(413)→A | Cys(138)→Tyr |
| 1 | R | C(425)→A | Thr(142)→Lys |
| 1 | R | 12-nt insertion between nt 444 and 445 | In-frame insertion between amino acids 148 and 149 |
| 1 | R | T(464)→G | Val(155)→Gly |
| 2 | R | T(515)→C | Leu(172)→Pro |
| 1 | R | T(524)→C | Met(175)→Thr |
a
S, sensitive; R, resistant.
b
M. bovis.
c
M. bovis BCG.