FIG. 1.

Expression construct used for the generation of PG-1 transgenic mice. (A) The expression fragment is released from the recombinant mammalian vector pcDNA3-PG-1-His by NruI and PvuII. The full sequence of porcine PG-1-His cDNA was located between the CMV promoter and the BGH polyA tail. (B) Schematic illustration of the precursor PG-1 peptide after intron splicing. It contains a signal peptide that can be released by signal peptidase. The cathelin domain is cleaved by elastase. Upon the removal of these two pieces, the mature PG-1 is the active form of the antimicrobial peptide.