Skip to main content
. 2008 Mar 3;52(5):1812–1819. doi: 10.1128/AAC.01530-07

FIG. 6.

FIG. 6.

Bactericidal activity of protein isolated from PG-1 transgenic and WT mice. (A) Bactericidal activity of neutrophil secretions isolated from the blood of transgenic (TG) mice (n = 8) and WT mice (n = 9) (RDU, radial diffusion unit). (B) Representative Western blot showing the PG-1-His protein in the medium of transgenic fibroblast culture after (lane 1) and before (lane 2) His purification and in transgenic fibroblast cell pellets (lane 3). (C) Representative results of an RDA: quadrants 1 and 10, ampicillin 100 μg/ml; quadrant 2, WT neutrophil secretion; quadrant 3, resuspension buffer of sample purification; quadrant 4, neutrophil elastase; quadrants 5 and 6, unpurified medium of cultured transgenic fibroblast medium digested with (quadrant 5) or without (quadrant 6) without neutrophil elastase; quadrants 7 and 8, His-purified medium of cultured transgenic fibroblast digested with (quadrant 7) or without (quadrant 8) neutrophil elastase; quadrants 9 and 10, transgenic fibroblast cell pellet digested with neutrophil elastase; quadrants 11 and 12, medium of cultured WT fibroblast medium digested with (quadrant 11) or without (quadrant 12) neutrophil elastase; quadrants 13 and 14, WT fibroblast cell pellet digested with (quadrant 13) or without (quadrant 14) neutrophil elastase.