TABLE 2.
Primer sequences
| Primer | Sequence (5′-3′) | Significant propertya |
|---|---|---|
| P1 | ATGACCATCCACAACCCCGAGGAAC | |
| P2 | AAGATATCTCAGCTCGCCTCTTCCTCGGTG | EcoRV restriction site |
| P3 | AAGATATCAGGTGGGTGAGATCTG | EcoRV restriction site |
| P4 | ATGCGGAGCAGGTCCTCGGTGTCCAG | |
| P5 | ACCGGCCCGGCCCCGGGGGCCTGCCCGGTCGTCGCGGAG | Exchange T for G |
| P6 | CTCCGCGACGACCGGGCAGGCCCCCGGCCGGGCCGGT | Exchange A for C |
| P7 | ATCATATGACCGCAGCGACACCG | NdeI restriction site |
| P8 | ATAAGCTTCTACGTCCCCTTCAGTTCG | HindIII restriction site |
| P9 | AACTGGCACACCCGCAACGGCGATGTG | |
| P10 | AGGTCCTTCGTGTGCGAGAACAGGTAG | |
| P11 | ACCTCCTCTTCGGCGACTGCATGGGCG | |
| P12 | AGGGTGGAAAGCCCTGCCATGATGTGG | |
| P13 | AAAGATCTGTGACCGACTGGATCCAGAG | BglII restriction site |
| P14 | AATAAGCTTTCACGCGGTGCTCCCCGGCTCCG | HindIII restriction site |
| P15 | AAGGATCCGTGAGCGGGCGGGGCG | BamHI restriction site |
| P16 | ATAAGCTTTCACTGCCACGTCCGGTC | HindIII restriction site |
| P17 | TACACCCACCTGCTGAAGTCC | |
| P18 | AAGATATCTCACCGCTCGCCCTCCTCGG | EcoRV restriction site |
| P19 | AAGATATCGTGAGAGACACCGGCCCGGC | EcoRV restriction site |
| P20 | TCACCGCGCACAGTGCCGCACG |
a
The sequences from which the properties are derived are underlined in the primer sequence.