TABLE 1.
Antimicrobial activities of mBD-14 and hBD-3 against various microorganisms
| Straina | MBCb (μM) of mBD-14 | LD90 (μM) of protein
|
|
|---|---|---|---|
| mBD-14 | hBD-3c | ||
| Gram-positive bacteria | |||
| Staphylococcus aureus ATCC 12600 | 1.2 | 0.08-0.15 | 0.15 |
| Staphylococcus aureus ATCC 33593 (MRSA) | 2.4 | 0.30 | 0.6 |
| Staphylococcus epidermidis ATCC 14990 | 2.4 | 1.2 | 0.15 |
| Streptococcus pyogenes ATCC 12344 | 1.2 | 0.15 | 1.2 |
| Enterococcus faecalis ATCC 29212 | 1.2 | 0.08-0.15 | |
| Enterococcus faecalis ATCC 51299 (VRE) | 2.4 | 0.30 | |
| Gram-negative bacteria | |||
| Escherichia coli ATCC 11775 | 0.60-1.2 | 0.08-0.15 | |
| Escherichia coli ATCC 25922 | 1.2 | 0.30 | 0.02 |
| Escherichia coli ESBL 4 (clinical isolate) | 0.60 | 0.15 | 0.15 |
| Salmonella enterica serovar Typhimurium ATCC 13311 | 1.2 | 0.15 | |
| Pseudomonas aeruginosa ATCC 10145 | 1.2-2.4 | 0.30 | 0.30 |
| Pseudomonas aeruginosa Va 6201/2000 (multiresistant; clinical isolate) | 4.8 | 0.60 | 0.15 |
| Burkholderia cepacia ATCC 25416 | >19 | >19 | >19 |
| Burkholderia cepacia ATCC 17759 | >19 | >19 | |
| Burkholderia stabilis NCTC 13011 | >19 | >19 | |
| Yeast | |||
| Candida albicans ATCC 24433 | 1.21 | 0.60 | |
a
MRSA, methicillin-resistant Staphylococcus aureus; VRE, vancomycin-resistant enterococcus.
b
MBC, minimal bactericidal concentration.
c
LD90s of hBD-3 were calculated based on the work of Sahly et al. (15).