FIG. 1.
P8 PGLC is on the surface of L. pifanoi amastigotes and binds to murine macrophages in a concentration- and time-dependent manner. (A) Indirect immunofluorescence of P8 PGLC staining on L. pifanoi amastigotes (see Materials and Methods). (B and C) Resident peritoneal macrophages were plated at 105 cells/well and incubated with 1 μg/ml P8 PGLC over a period of time (B) or with increasing concentrations of P8 PGLC at 4°C for 1 h (C). The level of binding was determined as indicated in Materials and Methods. Data are representative of three independent experiments. OD, optical density.