FIG. 4.

P8 PGLC induces the induction of inflammatory cytokines through TLR. MyD88^−/− (A), TLR2^−/− (B), TLR4^−/− (C) and WT (A, B, and C) (all C57BL/6 background) thioglycolate elicited-macrophages were stimulated with either P8 PGLC (1 μg/ml), LPS (1 μg/ml), PGN (5 μg/ml) in combination with IFN-γ, or IFN-γ alone or left unstimulated. After 48 h, supernatants were harvested and subsequently used to determine TNF-α levels. ELISAs were performed in duplicate, and data are representative of two independent experiments with similar results and are expressed as the mean and standard error.