FIG. 1.
Survival curves for D. melanogaster flies challenged with Pseudomonas isolates. D. melanogaster fly feeding is shown in panels A, C, and E, and fly nicking is shown in panels B, D, and F. The percent survival of PAO1 (*), PAK (▪), PA14 (▾), PA103 (▴), and medium control (⋄) was graphed over time in days (A) and hours (B). The Pseudomonas strains from acute infections graphed in panels C and D are Utah4 (▴), Utah3 (⧫), and ATCC 27853 (•). In panels E and F, the percent survival values for the CF isolates are indicated as follows: 14649 (*), 14673 (⧫), 14671 (⋄), 7307 (▪), 14684 (▴), 14670 (•), 14716 (□), 14683 (▵), 5588 (▾), 5552 (-⧫-), 14715 (▾), 5585 (○), 14661 (-×-), 14690 (-+-), 14703 (▪), 14651 (-•-), 14656 (-□-), 14655 (-▿-), 14672 (-▾-), 14685 (-▴-), 4384 (×), 14660 (-⋄-), 14717 (-*-), 14650 (|), 5166 (+), 5154 (-○-), and 6106 (-|-). Symbols without dashes indicate symbols connected by a solid line on the graph, and symbols with dashes indicate symbols connected by a dashed line on the graph. Fly mortality was measured hourly in the fly nicking assays and daily in the fly feeding assays using sets of 30 flies. The data are representative of the means of three or more replicate experiments performed in triplicate.