TABLE 1.
Evolution of M. paratuberculosis S-23 numbers in spleen, liver, and lungs as determined by luminometry and classical CFU counting
| Organ | Mouse | No. of bacteriaa
|
|||||
|---|---|---|---|---|---|---|---|
| 4 wk after infection
|
12 wk after infection
|
||||||
| Log10 RLU | Log10 CFU | Log10 CFU(h) | Log10 RLU | Log10 CFU | Log10 CFU(h) | ||
| Spleen | C57BL/6 | 5.29 ± 0.44 (5) | 5.56 ± 0.58 (5) | 5.39 ± 0.66 (5) | 4.59 ± 0.21 (5) | 5.80 ± 0.11 (5) | 5.47 ± 0.13 (5) |
| BALB/c | 5.16 ± 0.15 (4) | 5.63 ± 0.19 (4) | 5.66 ± 0.13 (4) | 5.23 ± 0.34 (4) | 6.20 ± 0.52 (3) | 6.02 ± 0.34 (3) | |
| C.D2 | 3.88 ± 0.10 (4) | 4.70 ± 0.17 (4) | 4.58 ± 0.19 (4) | 3.52 ± 0.12 (6) | 3.97 ± 0.38 (6) | 4.02 ± 0.28 (6) | |
| Liver | C57BL/6 | 5.59 ± 0.06 (5) | 6.68 ± 0.09 (5) | 6.52 ± 0.10 (5) | 3.65 ± 0.22 (5) | 5.48 ± 0.25 (5) | 5.24 ± 0.25 (5) |
| BALB/c | 5.87 ± 0.06 (4) | 6.62 ± 0.13 (4) | 6.38 ± 0.16 (4) | 5.33 ± 0.40 (3) | 7.07 ± 0.27 (3) | 6.50 ± 0.18 (3) | |
| C.D2 | 4.40 ± 0.14 (4) | 5.58 ± 0.19 (4) | 5.42 ± 0.24 (4) | 3.22 ± 0.10 (6) | 4.80 ± 0.19 (6) | 4.60 ± 0.34 (6) | |
| Lungs | C57BL/6 | 3.68 ± 0.17 (5) | 4.01 ± 0.36 (5) | 4.07 ± 0.48 (5) | 3.06 ± 0.07 (5) | 3.76 ± 0.63 (5) | 3.34 ± 0.51 (5) |
| BALB/c | 3.34 ± 0.02 (4) | 4.15 ± 0.66 (4) | 4.06 ± 0.70 (4) | 3.42 ± 0.71 (3) | 5.00 ± 0.62 (3) | 4.59 ± 0.73 (3) | |
| C.D2 | 2.90 ± 0.02 (4) | 4.41 ± 0.71 (4) | 4.26 ± 0.73 (4) | 2.95 ± 0.17 (6) | 2.85 ± 0.40 (6) | 2.89 ± 0.46 (6) | |
a
Organs from individual infected mice were homogenized, and the numbers of bacteria/organ were determined by luminometry or by plating the bacteria on Middlebrook 7H11 agar with [CFU(h)] or without (CFU) hygromycin. The data represent mean (± standard deviation) values for three to six mice (numbers are given in parentheses).