TABLE 2.
Gene expression in cervicovaginal tissuesa from uninfected control mice
| Group and gene | Product | Expression levelb |
|---|---|---|
| Proinflammatory cytokines | ||
| Il-1a | IL-1α | 2.67 ± 0.85 |
| Il-1b | IL-1β | 2.12 ± 0.69 |
| Il-1R1 | IL-1 receptor type I | 2.59 ± 0.92 |
| Il-6 | IL-6 | 2.03 ± 0.61 |
| Il-18 | IL-18 | 3.73 ± 0.42 |
| Il-21 | IL-21 | 1.69 ± 0.82 |
| Il-25 | IL-25 | 1.75 ± 0.79 |
| Anti-inflammatory cytokines | ||
| Il-1R2 | IL-1 receptor type II | 3.85 ± 0.63 |
| Il-9 | IL-9 | 2.25 ± 0.57 |
| Il-10Rb | IL-10 receptor, β | 4.69 ± 0.62 |
| Il-11 | IL-11 | 1.52 ± 0.46 |
| Il-11Ra1 | IL-11 receptor, α chain 1 | 2.18 ± 1.14 |
| Il-13 | IL-13 | 6.99 ± 1.22 |
| Il-13Ra1 | IL-13 receptor, α chain 1 | 1.69 ± 0.58 |
| Il-16 | IL-16 | 2.09 ± 0.63 |
| Il-20 | IL-20 | 2.15 ± 1.27 |
| T-cell growth factors | ||
| Il-2 | IL-2 | 3.60 ± 0.87 |
| Il-2Ra | IL-2 receptor, α chain | 1.00 ± 0.00 |
| Il-2Rb | IL-2 receptor, β chain | 2.81 ± 0.68 |
| Il-15Ra | IL-15 receptor, α chain | 2.46 ± 0.86 |
| TGF ligands | ||
| TGF-a | TGF-α | 3.13 ± 0.34 |
| TGF-b3 | TGF-β3 | 2.88 ± 0.75 |
| TNF ligands and receptors | ||
| Tnf | TNF | 3.90 ± 1.10 |
| Ltb | Lymphotoxin B | 6.06 ± 1.28 |
| LtbR | Lymphotoxin B receptor | 2.43 ± 0.71 |
| Chemokines | ||
| Ccl9 (MIP-1g) | Chemokine (C-C motif) ligand 9 | 1.33 ± 0.33 |
| Ccl3 (MIP-1a) | Chemokine (C-C motif) ligand 3 | 1.88 ± 0.79 |
| Ccl4 (MIP-1b) | Chemokine (C-C motif) ligand 4 | 1.47 ± 0.47 |
| Ccl7 (MCP-3) | Chemokine (C-C motif) ligand 7 | 2.80 ± 0.66 |
| Ccl12 (MCP-5) | Chemokine (C-C motif) ligand 12 | 1.34 ± 0.34 |
| Ccl8 (MCP-2) | Chemokine (C-C motif) ligand 8 | 1.00 ± 0.00 |
| Ccl11 (Eotaxin) | Chemokine (C-C motif) ligand 11 | 2.13 ± 0.87 |
| Ccl20 (MIP-3a) | Chemokine (C-C motif) ligand 20 | 2.79 ± 0.65 |
| Ccl21a (Scya21a) | Chemokine (C-C motif) ligand 21a | 4.64 ± 1.47 |
| Ccl25 (TECK) | Chemokine (C-C motif) ligand 25 | 3.71 ± 1.01 |
| Cxcl2 (MIP-2) | Chemokine (C-X-C motif) ligand 2 | 4.14 ± 1.02 |
| Cxcl9 (MIG) | Chemokine (C-X-C motif) ligand 9 | 2.00 ± 1.15 |
| Cxcl10 (IP-10) | Chemokine (C-X-C motif) ligand 10 | 1.64 ± 0.45 |
| Cxcl11 (IP-9) | Chemokine (C-X-C motif) ligand 11 | 1.18 ± 0.23 |
| Cxcl14 (MIP-2g) | Chemokine (C-X-C motif) ligand 14 | 1.73 ± 0.25 |
| Xcl1 (Lymphotactin) | Chemokine (C motif) ligand 1 | 2.24 ± 1.24 |
| Cx3cl1 (Fractalkine) | Chemokine (C-X3-C motif) ligand 1 | 2.04 ± 1.04 |
| Chemokine receptors | ||
| Ccr1 | Chemokine (C-C motif) receptor 1 | 1.00 ± 0.00 |
| Ccr5 | Chemokine (C-C motif) receptor 5 | 3.33 ± 0.83 |
| Ccr2 | Chemokine (C-C motif) receptor 2 | 3.82 ± 0.38 |
| Ccr7 | Chemokine (C-C motif) receptor 7 | 2.45 ± 0.68 |
| Ccr8 | Chemokine (C-C motif) receptor 8 | 1.66 ± 0.66 |
| Ccr9 | Chemokine (C-C motif) receptor 9 | 2.67 ± 1.06 |
| Cxcr3 | Chemokine (C-X-C motif) receptor 3 | 1.00 ± 0.00 |
| Cxcr1 | Chemokine (C-X-C motif) receptor 1 | 2.71 ± 1.00 |
| Cx3cr1 | Chemokine (C-X3-C motif) receptor 1 | 1.86 ± 0.56 |
| Cxcr4 | Chemokine (C-X-C motif) receptor 4 | 1.00 ± 0.06 |
a
To minimize interindividual variability within mouse groups, total isolated RNA was pooled from paired tissue samples at 24 h and 7 days after PBS injection.
b
Gene expression levels were scaled from 1 to 10 as described in Materials and Methods, and results are expressed as the mean expression level of the mRNA signal obtained from cervicovaginal tissues harvested 24 h and 7 days after PBS injection ± the SEM.