TABLE 3.
Changesa in gene expression in cervicovaginal tissues from miceb 24 h and 7 days after ivag infection with live recombinant AroA kanL1S or PhoPc kanL1S
| Group and gene | Product | 24 h
|
7 days
|
||
|---|---|---|---|---|---|
| AroA | PhoPc | AroA | PhoPc | ||
| Proinflammatory cytokines | |||||
| Il-1a | IL-1 α | + (*)c | + (*) | U | U |
| Il-1b | IL-1 β | + | + | + | + |
| Il-1R1 | IL-1 receptor type I | + | + | U | U |
| Il-21 | IL-21 | + | + | U | U |
| Anti-inflammatory cytokines | |||||
| Il-11 | IL-11 | + (*) | + | U | U |
| Il-11Ra1 | IL-11 receptor, α chain1 | + (*) | + | U | U |
| Il-13 | IL-13 | − | U | U | U |
| Il-13Ra1 | IL-13 receptor, α chain1 | + | U | U | U |
| Il-16 | IL-16 | + | − | + | + |
| T-cell growth factors | |||||
| Il-2 | IL-2 | − (*) | − (*) | U | U |
| Il-2Ra | IL-2 receptor, α chain | + | + | U | U |
| Il-2Rb | IL-2 receptor, β chain | + | U | U | U |
| Il-15Ra | IL-15 receptor, α chain | + | U | + | + |
| TGF ligands | |||||
| TGF-a | TGF-α | U | − | U | U |
| Chemokines | |||||
| Ccl9 | Chemokine (C-C motif) ligand 9 | + | + | U | U |
| Ccl4 | Chemokine (C-C motif) ligand 4 | + | U | U | U |
| Ccl12 | Chemokine (C-C motif) ligand 12 | U | U | + | + |
| Ccl8 | Chemokine (C-C motif) ligand 8 | + | U | U | U |
| Ccl21a | Chemokine (C-C motif) ligand 21a | U | − | U | U |
| Cxcl2 | Chemokine (C-X-C motif) ligand 2 | U | U | − | − |
| Cxcl10 | Chemokine (C-X-C motif) ligand 10 | + | U | U | U |
| Cxcl11 | Chemokine (C-X-C motif) ligand 11 | + | U | U | U |
| Cxcl14 | Chemokine (C-X-C motif) ligand 14 | + | − | U | − |
| Chemokine receptors | |||||
| Ccr1 | Chemokine (C-C motif) receptor 1 | + | + | U | U |
| Ccr5 | Chemokine (C-C motif) receptor 5 | U | + | U | U |
| Ccr7 | Chemokine (C-C motif) receptor 7 | + | U | U | U |
| Ccr8 | Chemokine (C-C motif) receptor 8 | + (*) | + | U | U |
| Ccr9 | Chemokine (C-C motif) receptor 9 | + | + | − | − |
| Cxcr3 | Chemokine (C-X-C motif) receptor 3 | + (*) | + (*) | U | U |
| Cxcr1 | Chemokine (C-X-C motif) receptor 1 | + | + (*) | U | U |
| Cxcr4 | Chemokine (C-X-C motif) receptor 4 | + (*) | + (*) | U | U |
a
Changes in gene expression in infected mice that exhibited a mean expression value at least 2 standard deviations out of the mean expression values obtained with PBS control mice and a change of >1.5-fold compared to control tissues. U, unchanged; + or −, up- or down-regulated, respectively.
b
Gene expression changes represent the mean value obtained from two pools of cervicovaginal tissues (two mice per pool).
c
(*), more-than-threefold change.