FIG. 2.

26- or 6-Y. pestis invades DCs. Gentamicin protection-, flow cytometry-, and fluorescence microscopy-based assays were used to determine the DC invasion rates of Y. pestis. E. coli strain K-12 (CS180 and CS1681) and the N. gonorrhoeae F62 lgtB mutant were utilized as controls, whose invasive nature has been reported recently (32, 61, 62). (A) Data from the gentamicin protection assay. (B) Data from flow cytometry. Labeled and unlabeled bacteria are shown in open and filled curves, respectively. (C and D) Adherence (C) and phagocytosis (D) data acquired from fluorescence microscopy assays. (A and D) *, P < 0.001, calculated by Student's t test, in the comparison of the invasion of DCs by 26- and 37-Y. pestis. n was nine samples for each test. The error bars indicate standard errors.