FIG. 3.

Cl₂MDP-liposome treatment during H. pylori infection reduces gastric inflammation. C57BL/6 mice were treated with either PBS (white symbols) or Cl₂MDP-liposomes (black symbols) from 8 days prior to the first dose of H. pylori until 10 days after the final dose of H. pylori. (A) Sections of gastric tissue from mice infected H. pylori for 3 months were stained with hematoxylin and eosin and examined for gastritis by light microscopy. Values for mononuclear cell infiltration, described as chronic inflammation, were estimated by using a six-point scale. Shown are representative images of PBS-treated (a to d) and Cl₂MDP-liposome-treated (e to h) mice showing inflammatory infiltrate (arrows) in the upper-body (a [score = 2.5] and e [score = 1.0]), mid-body (b [score = 2.0] and f [score = 1.5]), lower-body (c [score = 4.0] and g [score = 2.5]), and antrum (d [score = 3.0] and h [score = 2.0]) regions of the stomach. Original magnification, ×20. (B) Box plot showing the gastric inflammatory scores of Cl₂MDP-liposome (n = 10, hatched boxes) and PBS-treated (n = 10, white boxes), H. pylori-infected animals determined by histology 3 months after infection. Data are representative of two independently performed experiments.