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Double label immunofluorescence microscopy following incubation with anti-rTP0136 and anti-FlaA antibodies with Percoll-purified T. pallidum in the absence (A) or presence (B) of 0.1% Triton X-100 detergent. Antibody binding of anti-rTP0136 antibody was detected using Alexa Fluor 488 goat anti-rabbit IgG (green), and antibody binding of anti-FlaA was detected using Alexa Fluor 546 goat anti-rat IgG (red).
