FIG. 2.
Proteome reference maps of L. pneumophila supernatant subfractions. (A) SSP fraction. The two-dimensional reference map is divided into the following four sections: panel 1, upper left section (high Mr and low pI); panel 2, upper right section (high Mr and high pI); panel 3, lower left section (low Mr and low pI); and panel 4, lower right section (low Mr and high pI). (B) Two-dimensional reference map of the OMV fraction. Isolated protein fractions were focused on pH 3 to 10 IPG strips and separated by SDS-PAGE second-dimension gels. Gels were stained with Coomassie brilliant blue G-250. Proteins were identified following tryptic digestion and analysis of the resulting peptides by MALDI-TOF MS. Gene designations in the L. pneumophila Philadelphia-1 database (http://legionella.cu-genome.org/) are indicated. All MS-identified proteins are listed in Table S1 in the supplemental material.