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OMVs are not enough to kill L. pneumophila host cells. NCI-H292 alveolar epithelial cells (▪) and A. castellanii (A. c.) protozoan host cells (▴) were incubated with 50 μg (total protein) of OMVs. Cell suspensions without OMVs served as controls (□ and ▵). After 24 h Alamar blue was added. Then cell growth was monitored by examining Alamar blue reduction over 72 h at 24-h intervals.
