FIG. 2.

Immunoblots assessing OspD expression in various B. burgdorferi strains. (A) OspD antiserum reacts with a protein of the appropriate size (28 kDa) from a whole-cell lysate of the A3 wild-type (WT) strain, but not with whole-cell lysates from the OspD mutant strains (ΔD109 and ΔD117). The rOspD-thioredoxin fusion protein (∼42 kDa) was run in parallel as a positive control. (B) Individual serum samples from three mice infected by tick bite were incubated with immunoblots of purified rOspD protein and whole-cell lysates of B. burgdorferi wild-type and OspD mutant strains. A single, representative immunoblot is shown. Molecular mass standards (in kilodaltons) are indicated beside each panel.