FIG. 3.
Kinetics of IRF-1 and IRF-8 induction in Leishmania-infected DC. RNA isolated from uninfected DC (open) or DC infected with L. major (black bars) or L. donovani (gray bars) for 1, 2, 4, 6, 8, 12, and 16 h was analyzed for IRF-1 (A) and IRF-8 (B) transcript levels by quantitative real-time RT-PCR. The induction of IRF-1 and IRF-8 transcripts in Leishmania-infected DC was calculated using the comparative ΔΔCT method and normalized to GAPDH. Values are means ± SEM of triplicate culture wells. L. major-infected DC from all donors exhibited significant up-regulation of IRF-1 and IRF-8 mRNAs by 4 h postinfection (P ≤ 0.05).