FIG. 4.

Nuclear translocation of IRF-1 and IRF-8 in L. major-infected DC. Nuclear extracts obtained from DC infected with L. major (Lm) or L. donovani (Ld) for 1, 8, or 16 h were analyzed by Western blotting for IRF-1 (A) and IRF-8 (B). IFN-γ (1,000 U/ml)-stimulated DC were used as a positive control. Densitometry readings for each blot were normalized to lamin A/C. Western blots for one representative donor are shown. Values are means ± SEM of 3 to 12 donors. †, P ≤ 0.1; *, P ≤ 0.05. M, medium control.