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. 2008 Mar 3;76(5):2018–2024. doi: 10.1128/IAI.01409-07

FIG. 2.

FIG. 2.

Surface localization of Pfs25 in the ookinetes of TrPfs25Pb. In vitro-derived (A to C) and in vivo-derived (D to F) ookinetes were fixed with methanol and reacted with antibodies for IFA. (A and D) Ookinetes of WT P. berghei labeled with anti-Pfs25; (B and E) WT ookinetes labeled with anti-Pb28 antibody; (C and F) ookinetes of TrPfs25Pb labeled with anti-Pfs25. (G and H) WT parasites (merged image from FITC and 4′,6′-diamidino-2-phenylindole labels) (G) and transgenic parasites (FITC merged with 4′,6′-diamidino-2-phenylindole labels) (H) show IFA results with blood-stage parasites allowed to react with anti-Pfs25 antibodies. (I) RT-PCR results demonstrating expression of Pfs25 transcripts in blood stages of transgenic parasites (T, duplicate lanes) compared to wild-type parasites (W, duplicate lanes). Genomic DNA from P. falciparum 3D7 was used as a positive control.