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Eap induces the expression of proinflammatory cytokines by PBMCs. Cytokine production was measured with the Th1/Th2 cytometric bead array in PBMC supernatant and is expressed as the increased (fold) expression relative to the PBMCs incubated in medium alone. The native Eap protein from the Newman strain induces the expression of TNF-α and IL-6 (proinflammatory cytokines) by PBMCs at high levels.
