FIG. 3.

(A) Direct binding of CIDR-f protein to CD36/Fc. Neither the PFE1640w protein nor the DBP (negative control) show significant binding to CD36, with which the well was coated. Results are expressed as means and standard deviations from three independent experiments. (B) Peptide inhibition assay of CIDRα-CD36 binding. Three peptides, i.e., CD36:145-171, CD36:146-164, and a nonrelated peptide, were tested for their effects on inhibition of the interaction between CIDR-f and CD36/Fc. The percentage of binding inhibition was calculated as the ratio of OD₄₀₅ in the presence of competitor to OD₄₀₅ of the control sample (in the absence of peptide competitor). (C) Ability of loop deletion constructs to bind to CD36. (D) Binding of C-terminally truncated CIDR-f domains to CD36.