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. 2008 Feb 25;76(5):1837–1847. doi: 10.1128/IAI.00480-07

FIG. 5.

FIG. 5.

(A) Blockade of CD36-adherent PEs of strain 3D7 using recombinant CIDR-f proteins. Recombinant CIDR-f proteins at 1.56 μM to 12 μM were preincubated with HLECs or CHO-K1 cells in the binding buffer. After 30 min of incubation, both CD36-adherent and CSA-adherent PEs of strain 3D7 were added to each well and tested for binding. The average number of PEs bound to 100 HLECs or CHO-K1 cells in three microscopic fields (magnification, ×40) was calculated. The percentage of inhibition of parasite binding was compared to that for the untreated control without the addition of any proteins. (B) Blockade of CD36-adherent PEs of strains 3D7, HB3, and FCR3 using recombinant proteins CIDR-f, 1640-f chimera, PFE1640w, and CIDR-Δ106. A quantity of 6 μM of each recombinant protein was preincubated with HLECs or CHO-K1 cells in the binding buffer. The average number of PEs bound to 100 HLECs or CHO-K1 cells in three microscopic fields (magnification, ×40) was calculated. Standard deviations were determined from three independent experiments.