FIG. 3.
Effect of Mac-2 on ROS generation in human blood stimulated with opsonized latex beads (Ops LB). ROS generation was determined continuously over a 90-min period (A) in the presence of 5 μg/ml Mac-2M28 (▪) or Mac-2M28C257Y (□) or without antagonist (▴). Rates of ROS generation were determined for every 5 min as described above. (B) Inhibition of ROS peak production in the presence of Mac-2M8, Mac-2M8Y257C, Mac-2M28, Mac-2M28C257Y, or IdeSM1. ROS peak production is the average ROS production from 8 min to 45 min. All values are means ± standard deviations (n = 3). *, P < 0.05; **, P < 0.01; and ***, P < 0.001 (Student's t test).