FIG. 2.

Immunization using isolated surface complexes induced a subset of antigen-specific antibodies compared to immunization using whole outer membranes. Antigens in A. marginale isolated from bovine erythrocytes were detected with serum from immunized animals that was diluted 1:3,000. A different exposure is shown for outer membrane-immunized animal 53* to more clearly indicate the doublet at 100 kDa. No specific antibodies were detected in animals prior to immunization (shown for animal 72 [72P]) or in animals inoculated with adjuvant alone.