TABLE 2.
Composition of surface protein complexes in A. marginale cells isolated from erythrocytes
| Protein | Surface location basis (reference)a | PSORT prediction (score) | MASCOT ion scoreb
|
|||
|---|---|---|---|---|---|---|
| BS3 (11.4 Å) | DTSSP (12 Å) | Sulfo-EGS (16.1 Å) | Sulfo-SAND (18.5 Å) | |||
| Msp2 | Exptl (12, 24) | 1,127 | 1,951 | 858 | 116 | |
| Msp3 | Exptl (12, 24) | 1,087 | 1,204 | 858 | 206 | |
| Omp7 | Exptl (12)c | 166 | 338 | 66 | 99 | |
| PSORT | Outer membrane (0.309) | |||||
| Am854 | Exptl (12)c | 102 | 78 | 53 | ||
| PSORT | Outer membrane (0.790) | |||||
| Omp8 | PSORT | Outer membrane (0.414) | 100 | 103 | 58 | |
| OpAG2 | Exptl (11) | 166 | 113 | 51 | ||
| Am779 | PSORT | Outer membrane (0.936) | 53 | 87 | ||
| Msp1a | Exptl (24) | 100 | 101 | |||
| Msp4 | Exptl (24) | 98 | 78 | |||
| Omp1 | PSORT | Outer membrane (0.166) | 60 | 70 | ||
| Omp9 | PSORT | Outer membrane (0.530) | 257 | 135 | ||
| Am1011 | Not predicted | 55 | ||||
| Am780 | Not predicted | 63 | ||||
| Omp11 | PSORT | Outer membrane (0.795) | 64 | |||
| VirB10 | Exptl (12)c | 51 | 58 | |||
| Am1051 | Not predicted | 52 | ||||
| Am366 | Exptl (12)c | 51 | ||||
| Am712 | Not predicted | 50 | ||||
a
Surface exposure was determined based on experimental evidence (reference in parentheses) or a PSORT prediction for gram-negative bacteria (http://psort.nibb.ac.jp/form.html).
b
MASCOT ion scores that were greater than 47 for the linking agent (spacer arm lengths indicated in parentheses) and were significant (P < 0.0001). Included is the highest ion score from multiple samples.
c
Proteins were previously identified in whole outer membranes but with unconfirmed surface expression.