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. 2008 Mar 3;76(5):2219–2226. doi: 10.1128/IAI.00008-08

TABLE 3.

Identification of previously hypothetical A. marginale proteins

Gene identity MASCOT ion scorea % Sequence coverageb PSORT predictionc Host celld BLAST homologye
Am778f 940 42 Outer membrane, 0.925 ISE6 A. phagocytophilum, Ehrlichia spp., Rickettsia spp., Wolbachia spp.
Am779 87 17 Outer membrane, 0.936 RBC A. phagocytophilum, Ehrlichia spp., Rickettsia spp., Wolbachia spp.
Am1097 49 20 Periplasmic space, 0.944; outer membrane, 0.383 ISE6 A. phagocytophilum, Ehrlichia spp., Rickettsia spp., Wolbachia spp.
Am202 43 6 Inner membrane, 0.808; outer membrane, 0.790 RBC A. phagocytophilum, Ehrlichia spp., Neorickettsia sennetsu, Wolbachia spp.
Am862 120 20 Cytoplasm, 0.097 RBC A. phagocytophilum, Ehrlichia spp., N. sennetsu, Wolbachia spp.
Am780 63 21 Inner membrane, 0.145 RBC A. phagocytophilum, Ehrlichia spp., Wolbachia spp.
Am419 27 17 Inner membrane, 0.274 RBC A. phagocytophilum, Ehrlichia spp., Wolbachia spp.
Am391 85 39 Inner membrane, 0.164 RBC A. phagocytophilum, Ehrlichia spp., Wolbachia spp.
Am742 57 15 Inner membrane, 0.217 RBC A. phagocytophilum, Ehrlichia spp., Wolbachia spp.
Am660 153 28 Cytoplasm, 0.134 RBC A. phagocytophilum, N. sennetsu, Wolbachia spp.
Am1037 22 2 Periplasmic space, 0.938 ISE6 A. phagocytophilum, Ehrlichia spp.
Am1051 62 8 Inner membrane, 0.172 RBC A. phagocytophilum, Ehrlichia spp.
Am823 22 4 Inner membrane, 0.584 RBC A. phagocytophilum, Ehrlichia spp.
Am1048 21 1 Inner membrane, 0.423 ISE6 A. phagocytophilum, Ehrlichia spp.
Am375 85 21 Periplasmic space, 0.939 RBC, ISE6 A. phagocytophilum, Ehrlichia spp.
Am649 180 32 Inner membrane, 0.323 RBC, ISE6 A. phagocytophilum
Am347 28 9 Inner membrane, 0.179 RBC A. phagocytophilum
Am1248 24 19 Inner membrane, 0.542 RBC Ehrlichia spp.
Am630 40 5 Outside, 0.671 RBC Wolbachia spp.
Am540 41 5 Inner membrane, 0.688 RBC Nonconserved
Am368 18 1 Inner membrane, 0.391 RBC Nonconserved
Am550 21 3 Periplasmic space, 0.842 RBC Nonconserved
Am1359 21 10 Inner membrane, 0.501 ISE6 Nonconserved
Am346 1159 64 Plasma membrane, 0.600 RBC Nonconserved
Am1041 19 23 Inner membrane, 0.480 RBC Nonconserved
Am1225 77 11 Cytoplasm, 0.081 RBC Nonconserved
Am1226 69 41 Cytoplasm, 0.353 RBC, ISE6 Nonconserved
Am185 21 3 Cytoplasm, 0.179 RBC Nonconserved
Am265 35 10 Cytoplasm, 0.102 RBC, ISE6 Nonconserved
Am712 50 8 Inner membrane, 0.172 RBC Nonconserved
Am354 27 14 Inner membrane, 0.457 RBC Nonconserved
Am359 32 9 Inner membrane, 0.361 RBC Nonconserved
a

MASCOT ion scores of ≥18 and significant at P < 0.05 are reported. Data reported are for the highest ion score when a protein was identified in multiple samples.

b

Percentage of coverage by the identified peptide(s).

c

Highest score for subcellular location as predicted by PSORT prediction for gram-negative bacteria (http://psort.nibb.ac.jp/form.html).

d

Host cell from which A. marginale was isolated. RBC, red blood cells.

e

Included are organisms with proteins having an E value of less than 1e-5 when aligned with the A. marginale protein using NCBI BLAST (2.2.17).

f

Proteins shown in bold are those identified in surface-expressed complexes. The others were identified only in bacterial lysates.