FIG. 3.
Treatment of mice with rmIL-12. (A) Mice were administered increasing doses of rmIL-12 by IN instillation. IL-12 in the first ml of recovered BAL fluid was assayed by ELISA 24 h later. (B to D) Mice were challenged with 2 × 105 P. carinii organisms IT and then by IN inoculation of 0.4 μg rmIL-12 (or PBS) every 3 days. Mice then were sacrificed 2 or 4 weeks after infection. Each data point represents mean ± SD for at least six animals. * indicates P < 0.05 compared to PBS. (B) Infection burden assayed as rRNA copy number/lung. (C) Total and differential cell counts in lavage fluid at 2 weeks. (D) Concentrations of TNF-α, IL-12, IFN-γ, and MIP-2 in lavage fluid by ELISA at 2 weeks.