FIG. 5.

Lung lymphocyte phenotypes after gene transfer of rmIL-12 using an adenoviral vector. Control and CD4-depleted BALB/c mice were challenged with 2 × 10⁵ P. carinii organisms. Twelve days after challenge, mice were administered either 2 × 10⁸ PFU AdIL-12 or AdLuc by IT inoculation. Mice were sacrificed at 4 weeks, and lavaged lymphocytes were assayed by flow cytometry. Numbers of lavaged total T cells, CD4⁺ cells, CD4⁺ effector/memory cells, CD8⁺ cells, CD8⁺ effector/memory cells, and B cells are shown for CD4⁺ mice (A) and CD4-depleted mice (B). Negligible numbers of CD4⁺ T cells were observed in the CD4-depleted mice. Data are reported as mean ± SD, n = 5 or 6. * indicates P < 0.05 compared to AdLuc.