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Lack of characteristic virus-induced membranes in BHK21 cells transfected with mutant NS2A-I59N replicon RNA. (A) BHK-21 cells were electroporated with WT and mutant repPACβgal RNAs, fixed with 4% paraformaldehyde at 48 h, 72 h, and 96 h posttransfection, and stained with anti-NS3 antibodies, as described Materials and Methods. Virus-induced foci are highlighted by arrows. All images are taken at ×300 magnification except those for panels G to I, which were taken at ×680 magnification.
