TABLE 1.
Oligonucleotides used for construction of plasmids, RT-PCR, and sequencinga
| Primer name | Sequence (5′ to 3′)a |
|---|---|
| JNS1SacII-F | GGGCACCGCGGACCTGCCACCCGCACCb |
| J2A-R | CATGTCGGTCGATTTTCCAG |
| NS2A-F | TACAACGCTGACATGATTG |
| NS2A59N-F | CTATGTCAATCTGGTGGGGGCGGCCTTTG |
| NS2A59N-R | CACCAGATTGACATAGCGTAACACATCAG |
| NS2A59R-F | CTATGTCAGGCTGGTGGGGGCGGCCTTTG |
| NS2A59R-R | CACCAGCCTGACATAGCGTAACACATCAG |
| NS2A59S-F | CTATGTCAGTCTGGTGGGGGCGGCCTTTG |
| NS2A59S-R | CACCAGACTGACATAGCGTAACACATCAG |
| NS2A59T-F | CTATGTCACTCTGGTGGGGGCGGCCTTTG |
| NS2A59T-R | CACCAGAGTGACATAGCGTAACACATCAG |
| NS2A59V-F | CTATGTCGTTCTGGTGGGGGCGGCCTTTG |
| NS2A59V-R | CACCAGAACGACATAGCGTAACACATCAG |
| NS2A59Y-F | CTATGTCTATCTGGTGGGGGCGGCCTTTG |
| NS2A59Y-R | CACCAGATAGACATAGCGTAACACATCAG |
| NS2A149P-F | ACGTTCCCCACAACATCTAATGTGGTCGTC |
| NS2A149P-R | TGTTGTGGGGAACGTTATCGCTCTCAATA |
| NS2A198S-F | CAAAAAAGTCAGGAGCCAGTCTGCTATGTT |
| NS2A198S-R | CTCCTGACTTTTTTGCAGCTGCACTTC |
| PstIZeoframe-F | CAACCTGCAGAAGCCAAGTTGACCAGTGCCGc |
| NsiIZeo-R | GCGCATGCATCGTCCTGCTCCTCGGCCACd |
a
Introduced nucleotide mutations are shown in boldface, and inserted nucleotides are shown in boldface italics.
b
The SacII site is underlined.
c
The PstI site is underlined.
d
The NsiI site is underlined.