TABLE 1.

FRET between fluorescently N terminus-tagged CD81, CLDN1, and CLDN4^a

Donor	Acceptor	Cell type	Ntote	%FRET	Distance (nm)
Same protein type
g.CD81TRIPb	r.CD81TRIP	Huh-7.5	364	82	6.0 ± 1.8
	r.CD81TRIP	293T	102	69	5.8 ± 3.8
	r.CD81TRIP	293T-CLDN1	108	56	4.9 ± 2.9
	r.CD81TRIP	T84	159	54	5.6 ± 2.6
g.CLDN1BABEc	r.CLDN1BABE	293T	227	52	5.4 ± 1.6
Different protein typed
g.CD81TRIP	r.CLDN1TRIP	293T	142	59	6.2 ± 2.1
	r.CLDN1BABE	293T	147	58	5.8 ± 1.6
	r.CLDN1TRIP	293T-CLDN1	153	15*	1.1 ± 1.9***
g.CLDN1BABE	r.CD81TRIP	293T	245	47	5.8 ± 2.4
g.CD81TRIP	r.CLDN4BABE	293T	459	15*	7.4 ± 1.8

^aAreas of fluorescent protein colocalization were selected and imaged by confocal microscopy. All tests were performed using the Mann-Whitney test (*, P < 0.05; ***, P < 0.001).

^bHuh-7.5, 293T, 293T-CLDN1_TRIP and T84 cells were transduced with pTRIP vectors expressing g.CD81 and r.CD81.

^c293T cells were transduced with pBABE vectors expressing g.CD81 and r.CLDN1.

^d293T or 293T-CLDN1_TRIP cells were transduced with pTRIP and pBABE vectors expressing g.CD81, r.CD81, g.CLDN1, r.CLDN1, or r.CLDN4 as indicated.

^eN_tot, the number of colocalized regions analyzed.