FIG. 7.

RB binding motifs in pUL97 impact the inhibition of aggresome formation. COS7 cells were transfected with plasmids expressing pp65-GFP, plasmids expressing a UL27-negative control, and plasmids expressing UL97, with the point mutations as labeled. (A) The graph depicts the percentage of cells containing visible aggregates. The values shown are the averages of six separate experiments, with the standard deviations (error bars) shown; the exception was the double mutant, for which values were determined twice. (B) Recombinant viruses with mutations in the UL97 kinase domain or the RB binding domains also form large aggregates. Viruses with point mutations in the amino acids shown were used to infect confluent HFF cells and were harvested 8 days following infection. Shown are fluorescent phase-contrast images of infected cells stained with an antibody to pp65 (green) to confirm viral infection.