TABLE 1.
Effect of coexpressed viral proteins on pp65-GFP expression and aggregation in COS7 cells
| Proteins expresseda | pp65 GFP fluorescenceb | % Cells containing aggregatesc |
|---|---|---|
| pp65-GFP, ppUL44 | 100 ± 3 | 67 ± 7 |
| pp65-GFP, ppUL97-V5 | 94 ± 1 | 10 ± 4d |
| pp65-GFP, UL97-V5 (K355M) | 104 ± 1 | 57 ± 16 |
| pp65-GFP, IE1 | 125 ± 1 | 84 ± 9d |
| pp65-GFP, pp71-V5 | 116 ± 2 | 92 ± 5d |
| pp65-GFP, pUL69-V5 | 93 ± 6 | 58 ± 9 |
a
Expression of protein from cotransfected plasmids was confirmed by fluorescence of pp65-GFP and antibody staining with antibodies to the second protein and Texas Red secondary antibody.
b
Fluorescence of cells expressing pp65-GFP was averaged from triplicate transfections and converted to percent control, with the standard deviations shown.
c
The percentages of cotransfected cells exhibiting pp65-GFP nuclear aggregates from four experiments were averaged, with the standard deviations shown.
d
Values shown were significantly different (P < 0.05) in a comparison with the control (pp65-GFP, ppUL44).