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. 2008 Mar 5;82(10):5054–5067. doi: 10.1128/JVI.02174-07

TABLE 1.

Effect of coexpressed viral proteins on pp65-GFP expression and aggregation in COS7 cells

Proteins expresseda pp65 GFP fluorescenceb % Cells containing aggregatesc
pp65-GFP, ppUL44 100 ± 3 67 ± 7
pp65-GFP, ppUL97-V5 94 ± 1 10 ± 4d
pp65-GFP, UL97-V5 (K355M) 104 ± 1 57 ± 16
pp65-GFP, IE1 125 ± 1 84 ± 9d
pp65-GFP, pp71-V5 116 ± 2 92 ± 5d
pp65-GFP, pUL69-V5 93 ± 6 58 ± 9
a

Expression of protein from cotransfected plasmids was confirmed by fluorescence of pp65-GFP and antibody staining with antibodies to the second protein and Texas Red secondary antibody.

b

Fluorescence of cells expressing pp65-GFP was averaged from triplicate transfections and converted to percent control, with the standard deviations shown.

c

The percentages of cotransfected cells exhibiting pp65-GFP nuclear aggregates from four experiments were averaged, with the standard deviations shown.

d

Values shown were significantly different (P < 0.05) in a comparison with the control (pp65-GFP, ppUL44).

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