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. 2008 Mar 19;82(10):5079–5083. doi: 10.1128/JVI.01958-07

TABLE 2.

Infectivity and virulence of Y98F mutant virus in mice

Virus 50% mouse infectious dosea (log10 PFU) Inoculum (PFU) Lung viral titer (log10 PFU/ml) at day 3b Mean maximum % wt lossc
WT 1.5 106 6.4 21.4
105 6.2
104 6.3
Y98F 4.5 106 3.5 1.2
105 1.8
104 2.1
a

Mice (n = 2) were infected i.n. with serial dilutions of virus from 106 to 101 PFU. Three days later, lungs were harvested and titers were determined individually in MDCK cells. The 50% mouse infectious dose was determined as the highest dilution of virus which infected mice.

b

Mice were infected i.n. with 106 to 104 PFU of WT or mutant virus. Lungs from three mice per inoculum group were collected and pooled, and titers were determined on MDCK cells.

c

A separate group of four mice was infected with 106 PFU of virus (each mouse) and was monitored for weight loss daily for 14 days. Mice infected with WT virus and Y98F virus exhibited maximum weight loss on days 8 and 4 postinfection, respectively. One mouse infected with WT virus succumbed on day 8.