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Stability of the FL BAC plasmid in E. coli. DH10B cells containing the FL BAC plasmid were passaged every day at a ratio of 1:100 (vol/vol) for 20 consecutive days. On the indicated days, BAC DNA from the culture was prepared. Finally, BAC DNA samples collected at various intervals were compared with parental FL strain and FL BAC strain DNA by SacI digestion and agarose gel electrophoresis. Marker sizes (MS) are indicated on the left.
