FIG. 5.

Structural analysis of the KHV FL BAC galK recombinant plasmid. The KHV FL BAC plasmid, the derived FL BAC ΔORF16 plasmid, and the genome of the KHV FL BAC ΔORF16-excised strain were analyzed by SacI restriction (agarose gel) and further tested by Southern blotting using a probe corresponding to ORF16. The KHV FL strain was used as a control. White and black arrowheads and open arrowheads indicate restriction fragments containing ORF16 and the galK cassette, respectively. The gray arrowhead indicates a restriction fragment containing the TK ORF. Marker sizes (MS) in kilobases are indicated on the left.