FIG. 6.

Cumulative survival rates of carp infected with FL BAC plasmid-derived strains. (A) On day 0, four groups, each consisting of 10 koi carp (with the exception of mock-infected groups, consisting of 13 carp), were inoculated by IP injection with mock-infected culture medium and culture medium containing 3 × 10² PFU of FL, FL BAC-excised, and FL BAC revertant strains. On day 32 pi, surviving fishes were challenged by IP injection with the parental FL strain. (B) On day 0, four groups, each consisting of 10 koi carp (with the exception of mock-infected groups, consisting of 13 carp), were inoculated by IP injection with mock-infected culture medium and culture medium containing 3 × 10² PFU of FL, FL BAC-excised, and FL BAC ΔORF16-excised strains. On day 27 pi, surviving fishes were challenged by IP injection with the parental FL strain. Percentages of surviving carp are expressed according to days pi. The results presented are representative of three independent experiments.