FIG. 6.

Immunoprecipitation of virus particles from control and CD63 knockdown MDM. Cell-free supernatants from control and CD63 knockdown MDM-derived viruses were incubated at 37°C for 1 h with antibodies against CD63, VSV-G (negative control), and Env (positive control). The virus-antibody mixture was incubated with Pansorbin cells, and viruses were precipitated by centrifugation. The supernatants, containing the unprecipitated viruses, were used to infect TZM-β-gal indicator cells. β-Gal activity was measured 24 h after infection and was expressed as arbitrary units (au). Bars represent averages of values from six different dilutions per sample from a representative experiment. Error bars represent the standard errors of the means. noAb, untreated sample.