FIG. 7.
Colocalization of the HIV matrix protein with CD63 on HIV-infected MDM. Semithin cryosections from human MDM samples infected with HIVBaL for 7 days (A), 14 days (B), or 20 days (C) were stained with a rabbit antiserum against HIV p17 and a mouse MAb to CD63, followed by anti-rabbit Alexa Fluor 594 and anti-mouse Alexa Fluor 488. Sections were examined with an Axioskop fluorescence microscope. The HIV-containing structures (red, left column) costained strongly with CD63 (green, center column), giving a yellow color in the merged images (right column). This distribution was seen for MDM cultures infected for various lengths of time, in single infected cells or syncytia, and for virus-containing compartments of various sizes and morphologies. By contrast, MDM samples infected with a passaged stock of HIV-1BaL for 8 days (D) or 13 days (E) did not accumulate CD63 in the virus-containing compartment, which appears red even in the merged images (right column). Scale bars = 10 μm.