TABLE 1.
Analysis of infectious virus production from control and CD63 knockdown MDM at 6 and 10 dpi
| dpi | Experiment no. | p24 level (ng/ml) for:
|
Infectivity level (105 FFU/ml) for:
|
Infectious unit per particleb for:
|
|||
|---|---|---|---|---|---|---|---|
| Control | KDa | Control | KD | Control | KD | ||
| 6 | 1 | 6.21 | 7.31 | 5.19 | 5.54 | 0.0167 | 0.0152 |
| 2 | 3.06 | 3.67 | 3.32 | 3.03 | 0.0217 | 0.0165 | |
| 3 | 4.94 | 2.73 | 4.66 | 3.89 | 0.0189 | 0.0284 | |
| 4 | 13.17 | 7.62 | 17.40 | 8.35 | 0.0264 | 0.0219 | |
| 5 | 11.59 | 9.60 | 7.62 | 4.96 | 0.0132 | 0.0103 | |
| 10 | 1 | 10.20 | 13.40 | 3.17 | 4.85 | 0.0062 | 0.0072 |
| 2 | 5.77 | 4.50 | 1.86 | 1.88 | 0.0064 | 0.0083 | |
| 3 | 3.91 | 2.81 | 1.56 | 1.16 | 0.0080 | 0.0083 | |
| 4 | 10.50 | 6.78 | 4.46 | 3.88 | 0.0085 | 0.0114 | |
| 5 | 13.09 | 8.03 | 4.02 | 4.03 | 0.0061 | 0.0100 | |
a
KD, CD63 knockdown MDM.
b
The number of infectious units per particle was calculated from the infectivity in FFU/ml and the p24 levels, assuming 5,000 Gag molecules per virion (4).