TABLE 2.
Analysis of infectious virus production from control and CD63 knockdown MDM at 6 dpi
| Experiment no. | p24 level (ng/ml) for:
|
Infectivity level (105 FFU/ml) for:
|
Infectious unit(s) per particleb (10−3)
|
Proviral DNAc (au) for:
|
||||
|---|---|---|---|---|---|---|---|---|
| Control | KDa | Control | KD | Control | KD | Control | KD | |
| 6 | 8.74 | 8.13 | 0.07 | 0.07 | 0.15 | 0.17 | 50.3 | 41.0 |
| 7 | 7.58 | 7.65 | 0.22 | 0.22 | 0.58 | 0.56 | 55.9 | 45.9 |
| 8 | 10.55 | 9.24 | 1.97 | 1.92 | 3.74 | 4.15 | 84.0 | 83.1 |
| 9 | 8.08 | 5.97 | 1.95 | 2.04 | 4.84 | 6.82 | 66.5 | 69.3 |
a
KD, CD63 knockdown MDM.
b
The number of infectious units per particle was calculated from the infectivity in FFU/ml and the p24 levels, assuming 5,000 Gag molecules per virion (4).
c
Proviral DNA is expressed as arbitrary units (au) based on the ratio of the integrated HIV-1 DNA level to the β-globin gene level.