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. 2008 Mar 5;82(10):4812–4822. doi: 10.1128/JVI.02570-07

FIG. 2.

FIG. 2.

Kinetics of m38.5 expression as shown by Western blotting. (A) Lysates of cells infected with the indicated viruses were harvested 24 hpi and immunoblotted with an anti-HA antibody that recognized the tagged version of m38.5 with a size of approximately 25 kDa. The MCMV IE1 protein was detected with an IE1-specific antibody. m38.5HA-kan, MCMV-m38.5HA-kan; m38.5HA, MCMV-m38.5HA; Δm38.5HA, MCMVΔm38.5HA; Δm38.5, MCMVΔm38.5; ΔM37-m39, MCMVΔM37-m39. (B) Cells infected with MCMV-m38.5HA were harvested at the indicated hours postinfection. The MCMV proteins IE1 and E1 and the late glycoprotein B (gB) were detected with specific antibodies. The m38.5 protein was detected with an anti-HA antibody. Immediate-early and early proteins were expressed in the presence of PAA. M, mock-infected cells. (C) Cells were mock infected (M) or infected (I) with MCMV-m38.5HA for 8 h. Viral protein expression was blocked in the presence of cycloheximide (C) or actinomycin D (A). Immediate-early proteins were expressed selectively after release from the cycloheximide block after 4 h and incubation with actinomycin D for another 4 h (C▸A). In all experiments, β-actin served as a loading control.