FIG. 3.

MCMVΔm38.5 induces nuclear DNA fragmentation. Murine 10.1 fibroblasts were infected at an MOI of 0.01 with wt MCMV or MCMVΔm38.5 (Δm38.5), both of which express GFP. GFP expression and nuclear DNA fragmentation (detected using a TUNEL assay) 3 days after infection were visualized by fluorescence microscopy. Nuclei were stained with DAPI. Cells infected with MCMVΔm38.5 are also shown at a higher magnification (third row).