FIG. 4.
Replication kinetics of wt and mutant MCMVs. (A) Single-step growth curves for MCMVs in 10.1 fibroblasts infected at an MOI of 5. m38.5HA-kan, MCMV-m38.5HA-kan; m38.5HA, MCMV-m38.5HA; Δm38.5, MCMVΔm38.5; Δm38.5HA, MCMVΔm38.5HA; ΔM37-m39, MCMVΔM37-m39. (B) Multistep growth curves for MCMVs in 10.1 fibroblasts infected at an MOI of 0.01. Infected cells were incubated with (open symbols) or without (black symbols) the daily exchange of the cell culture medium. TCID50, 50% tissue culture infective dose.