FIG. 6.
Construction of rescue mutants expressing m38.5 or Bcl-XL. (A) Sequences expressing HA-tagged versions of m38.5 and Bcl-XL driven by a phosphoglycerate kinase promoter (PGKp) were inserted into the nonessential region of the MCMVΔM37-m39 genome between the m02 and m06 genes. m38.5HA, gene expressing HA-tagged m38.5; HABclXL, gene expressing HA-tagged Bcl-XL. (B) This insertion results in the loss of a 2.16-kb HindIII fragment and the appearance of a new 1.25-kb fragment in the rescue mutants and an additional 0.96-kb fragment in RBcl-XL. Band patterns for two clones of each rescue mutant are shown. MW, molecular size standard; ΔM37-m39, MCMVΔM37-m39. (C) Detection of HA-tagged proteins (m38.5 and Bcl-XL) and the MCMV E1 protein in infected 10.1 cells 24 hpi. m38.5HA, MCMV-m38.5HA; Rm38.5(HA) and RBclXL(HA), Rm38.5 and RBcl-XL mutants expressing HA-tagged proteins.