FIG. 7.
The insertion of the m38.5 or Bcl-XL gene eliminates the defects caused by the deletion of the m38.5 gene. (A and B) The levels of viability of infected 10.1 cells at 24 and 96 hpi (A) and of infected STS-, MG132-, or TNF-α-treated 10.1 cells at 24 hpi (B) were measured by an MTT assay. In all experiments, the 24-hpi values for untreated cells were normalized to 100%. Δm38.5, MCMVΔm38.5; −, no treatment. (C) Multistep growth kinetics of wt and mutant MCMVs in 10.1 fibroblasts (MOI, 0.01). TCID50, 50% tissue culture infective dose; ΔM37-m39, MCMVΔM37-m39.