TABLE 1.
Interaction of Tcoil and CMV 1a in yeast cells
| GAL4 BD vector | GAL4 AD vector | Result for yeast cells with indicated vector(s) in assay(s) for:
|
|
|---|---|---|---|
| His | β-Galactosidase activityd | ||
| pAS2-1::CMV 1a | Nonea | − | − |
| Nonea | pACT2::Tcoil | − | − |
| pAS2-1::CMV 1a | pACT2 | − | − |
| pAS2-1 | pACT2::Tcoi1 | − | − |
| pAS2-1::CMV 1a | pACT2::Tcoil1 | + | + (33,000 ± 1,000) |
| pAS2-1::CMV 1a-MTb | pACT2::Tcoi1 | + | + (35,000 ± 2,000) |
| pAS2-1::CMV 1a-Helc | pACT2::Tcoil1 | − | − |
a
No coexpressed vector.
b
Encodes the MT domain of CMV 1a protein.
c
Encodes the Hel domain of CMV 1a protein.
d
The colony-lift filter assay and a liquid culture assay with o-nitrophenyl-β-d-galactopyranoside as the substrate were used to evaluate β-galactosidase activity. +, blue colonies resulting from positive β-galactosidase activity; −, white colonies resulting from negative β-galactosidase activity (same as that in the buffer control). Values are means ± standard deviations and are expressed as Miller units.